Interaction between mitochondrion and apicoplast in different stages of schizogony. (A) Micrograph images of contact sites between the apicoplast (yellow) and mitochondrion (red) in early-stage schizont, indicated by orange arrows. Nuclei are marked in teal. Scale bars, 0.5 μm. (B) 3D rendering of the mitochondrion (red, 50% opacity) and apicoplast (yellow), with contact sites indicated by orange arrows. (C) Micrograph images of mid- and fully segmented schizonts showing contact sites where apicoplast and mitochondrion are aligned over the total apicoplast length (black arrows). Red arrow indicates where the basal end of the apicoplast is in contact with the bulbous part of the mitochondrion at the merozoite entrance. Scale bars, 1 μm. (D) 3D rendering of apicoplast and mitochondrion in different stage schizonts. Blue arrows indicate where the basal end of an apicoplast interacts with the end of a mitochondrial branch. The number between brackets indicates schizont ID number (Table S3).