Mitochondrial division stages in ABS schizogony. Fluorescent imaging of MitoRed parasites in different mitochondrial division stages (described on the left). Images are representatives of the 17 parasites that were analyzed in the second independent experiment. Mito-mScarlet signal is shown in magenta and DAPI (DNA) in blue. Images are maximum-intensity projections of Z-stacks taken with an Airyscan confocal microscope. The fifth column shows the 3D image of the fluorescent mito-mScarlet signal, while the sixth column shows the 3D visualization of the segmented mitochondrial signal. The color of the mitochondrial fragment represents the size of this fragment, as is shown in the color bar at the bottom. Scale bars, 2 μm.