Figure 3.
Mitochondrial dynamics during ookinete development. (A) Live imaging of MitoRed ookinetes 1 day after mosquito feed. Different stages of ookinete maturation (II–V) were distinguished based on description by Siciliano et al. (2020). Cells were stained with an Alexa Fluor 488-conjugated anti-Pfs25 antibody to visualize the parasite outline (green). Images are maximum intensity projections of Z-stacks (30 slices, 185 nm interval) taken with an Airyscan confocal microscope. Scale bars, 2 μm. (B) 3D visualization of different ookinete maturation stages. The mito-mScarlet fluorescent signal is segmented based on manual thresholding. Two smaller images in the upper right corner of stages II–III and stage III are crops of the mitochondrial fluorescent signal with increased brightness and contrast. Scale bars, 1 μm.

Mitochondrial dynamics during ookinete development. (A) Live imaging of MitoRed ookinetes 1 day after mosquito feed. Different stages of ookinete maturation (II–V) were distinguished based on description by Siciliano et al. (2020). Cells were stained with an Alexa Fluor 488-conjugated anti-Pfs25 antibody to visualize the parasite outline (green). Images are maximum intensity projections of Z-stacks (30 slices, 185 nm interval) taken with an Airyscan confocal microscope. Scale bars, 2 μm. (B) 3D visualization of different ookinete maturation stages. The mito-mScarlet fluorescent signal is segmented based on manual thresholding. Two smaller images in the upper right corner of stages II–III and stage III are crops of the mitochondrial fluorescent signal with increased brightness and contrast. Scale bars, 1 μm.

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