Mitochondrial association with axonemes in activated male gametocytes. (A) Exflagellation events in MitoRed and NF54 parasites 20 min after activation in four cultures in two independent experiments. Unpaired t test showed no significant difference. (B) Three examples of activated males where the mitochondria (magenta) localize closely to the axonemal tubulin (green) in MitoRed parasites. Left images are single slices, right images are maximum intensity projections (MIPs). (C) Exflagellating MitoRed male gametocyte with apposition of the mitochondria with the axonemal tubulin. Top images are single slices and crops from the bottom image, which is a MIP. Scale bars, 2 μm.