Mitochondrial morphology in stage IV and V male and female gametocytes. (A and B) Immunofluorescence assay on male and female MitoRed gametocytes stage IV (A) and stage V (B), stained with anti-β-tubulin (green) and DAPI (blue). Male (M) and female (F) gametocytes are distinguished based on the intensity of the tubulin signal (males high, females low). Images are maximum-intensity projections of Z-stacks taken with an Airyscan confocal microscope. Scale bars, 2 μm.