Figure 2.
Mitochondrial dynamics during gametocyte development and activation. (A) Immunofluorescence assay on MitoRed gametocytes stages IIa, IIb, III, IV, and V, stained with anti-β-tubulin (green) and DAPI (DNA, blue). The mito-mScarlet signal is shown in magenta. In stages IV and V, male (M) and female (F) gametocytes are distinguished based on the intensity of the tubulin signal (males high, females low). (B) Immunofluorescence assay on MitoRed parasites during different stages of gametocyte activation (2, 5, 10, and 20 min after activation). (C) Immunofluorescence assay on MitoRed exflagellating male gamete 20 min after activation. (A–C) Images are maximum intensity projections of Z-stacks (41 slices, 150-nm interval) taken with an Airyscan confocal microscope. (D) 3D visualization of male and female MitoRed parasites 2, 5, 10, and 20 min after activation. The mito-mScarlet fluorescent signal is segmented based on manual thresholding. Scale bars, 2 μm.

Mitochondrial dynamics during gametocyte development and activation. (A) Immunofluorescence assay on MitoRed gametocytes stages IIa, IIb, III, IV, and V, stained with anti-β-tubulin (green) and DAPI (DNA, blue). The mito-mScarlet signal is shown in magenta. In stages IV and V, male (M) and female (F) gametocytes are distinguished based on the intensity of the tubulin signal (males high, females low). (B) Immunofluorescence assay on MitoRed parasites during different stages of gametocyte activation (2, 5, 10, and 20 min after activation). (C) Immunofluorescence assay on MitoRed exflagellating male gamete 20 min after activation. (A–C) Images are maximum intensity projections of Z-stacks (41 slices, 150-nm interval) taken with an Airyscan confocal microscope. (D) 3D visualization of male and female MitoRed parasites 2, 5, 10, and 20 min after activation. The mito-mScarlet fluorescent signal is segmented based on manual thresholding. Scale bars, 2 μm.

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