Fewer ER junctions are present at the cell periphery in PINK1-depleted cells. (A) siCtrl and siPINK1-treated cells transfected with Akita-sfGFP were quantitated as described in the methods. (B) Frames from representative 3D reconstructions of Z stacks of siCtrl (22 s in Video 2) and siPINK1-treated (23 s in Video 3) cells. (C) Compressed Z stacks of siCtrl and siPINK1-treated cells transfected with GFP-SEC61. A total of 27 slices were compressed for the siCtrl, and 19 slices for siPINK1-treated cells. (D) Left, representative images of LNPK-GFP in siCtrl and siPINK1-treated cells. Middle, the average number of LNPK puncta/cell. The data was normalized as described in the methods. Right, blots showing LNPK levels. GAPDH was used as a loading control. (E) Left, representative images used in the quantitation shown on the right. (F) Left, representative images of cells containing Akita-sfGFP with insets used in the quantitation (right) of junctions. (G) Same as E only, cells were transfected with mCherry-Sec61. Error bars represent SEM, n = 3 independent experiments. The results were quantified from 93 to 116 cells in A, 78–82 cells in D, 84–85 cells in E, 27–32 cells in F, and 51–59 cells in G. * (P < 0.05), **(P < 0.01), Student’s unpaired t test. Source data are available for this figure: SourceData F6.