Related to Fig. 1 . FAM134B and CALCOCO1 are not required for the delivery of LNPK puncta to lysosomes. (A) Left, control and siSEC24C-treated cells transfected with LNPK-GFP and mCherry-RTN3L. Arrowheads mark colocalizing puncta. Right, quantitation of percent LNPK puncta colocalizing with RTN3L puncta. (B) Bottom, same as Fig. 1 F, only siFAM134B and siCALCOCO1-treated cells were examined. The control is the same as in Fig. 1 F as the samples were all assayed at the same time. Bottom right, western blot analysis was performed with control and FAM134B-depleted lysates. GAPDH was used as a loading control. Error bars in A and B represent SEM, n = 3 independent experiments. The results were quantified from 40 to 50 cells in A, and 63–87 cells in B. NS: not significant (P ≥ 0.05), * (P < 0.05), **(P < 0.01), Student’s unpaired t test. Source data are available for this figure: SourceData FS5.