Related to Fig. 1 . RTN3L and SEC24C are delivered to lysosomes during basal autophagy. (A) Ctrl or SEC24C-depleted cells were treated for 4 h with Baf or Baf + MRT68921. Arrowheads mark RTN3L fragments delivered to lysosomes. The relative pixel intensity for each condition is the mean intensity of YFP-RTN3L in the pixels that overlap with LAMP1-mCherry for the images shown. The control for each condition was without Baf (DMSO only) and set to 1.0. MRT68921 was used to block autophagosome formation. (B) Same as A, only cells were depleted of RTN3L and the delivery of YFP-SEC24C to lysosomes was examined. Arrowheads mark SEC24C in lysosomes. Error bars represent SEM, n = 3 independent experiments. The results were quantified from 84 to 112 cells in A, and 88–128 in B. NS: not significant (P ≥ 0.05), * (P < 0.05), **(P < 0.01), Student’s unpaired t test.