A partnership of fam177a1 and vps13b in zebrafish. (A) Alphafold2 prediction of human VPS13B and zebrafish Vps13b. (B) Representative images of WT and fam177a1a;fam177a1b DKO (fam177a1DKO) zebrafish embryos that are either uninjected or injected with vps13b gRNAs/Cas9. The left panel shows embryos treated with DMSO at 6 hpf until 25 hpf whereas right panel shows embryos treated with 0.8 µg/ml BFA at 6 hpf until 25 hpf. (C) Quantifications of tail length results shown in B. Data are shown as means ± SEM, Unpaired, two-tailed t tests. n.s., not significant. ****P < 0.0001; ***P < 0.001; **P < 0.01.