CYRI controls border cell cluster cohesion and migration. (A and A′) Quantification of border cell cluster defects (lagging border cells and delayed border cell clusters) under the control of the (A′) c306-Gal4 and (A′) E132-Gal4 (upd-Gal4) driver. wild type; cyri RNAi #1; cyri RNAi #2; CYRI-WT OE; CYRI-R163/164D; wave RNAi and WAVE-Myr OE. N numbers are indicated. Quantified phenotypes are indicated by the colored legend in the middle. All genotypes were compared against WT using the chi-square test, P values: ns > 0.12, ***<0.001. At least three independent experiments for each genotype were performed. (B–D) Maximum intensity projections of stage 10A egg chambers of the indicated genotypes with DNA (DAPI, blue) and F-actin (red); anterior is to the left. Detailed views of the boxed area are shown on the right. (B) wild type; (C) CYRI overexpression (OE), yellow arrowhead marks nuclei of delayed border cell cluster; (D) RNAi-mediated suppression of wave function, yellow arrowhead marks delayed border cell cluster, whereas white arrowhead marks a lagging border cell.