Suppression of cyri function promotes epidermal wound closure. (A) Quantification of increased lamellipodial area upon expression of two different cyri RNAi transgenes (#1 and #2, n = 5), wave RNAi (n = 16), cyri; wave double RNAi (n = 12), CYRI^WT (n = 15) under the epidermis-specific A58-Gal4 driver. Note that wild type control (n = 13) and wave RNAi are the same as in Fig. 4, F and G and CYRI^WT is the same as Fig. 5 E′, as the data belong to the same dataset. Lamellipodia size was measured every 5 min and normalized to the initial size of the unwounded cell. The two-way ANOVA analysis with Dunnett correction was used and P values were obtained: P value: 0.12 (ns), 0.033 (*), 0.002 (**), <0.001 (***). (B–D) Multiple-cell ablation experiments in the abdominal epidermis of wild type and trans-heterozygous cyri mutant pupae (cyriΔ11/Df[ED4457]), marked by the expression of Lifeact-EGFP under the control of the A58-Gal4 driver. The data shown represent the wound area at the cell surface to illustrate the effects of rapid ingrowth of lamellipodia on wound size (B) Quantification of wound closure in wild type control (black) and trans-heterozygous cyri mutant pupae (purple). Wound size was measured every 10 min and normalized to the initial size of the unwounded cell, (n = 5). To evaluate statistical significance, the two-way-ANOVA Bonferroni post test, (P < 0.05) was used. Error bars represent SD. (C and D) Frames of spinning disc microscopy videos of 18 h APF old epidermis expressing a Lifeact-EGFP transgene under the control of the A58-Gal4 driver. The genotypes are indicated. Red asterisks mark ablated epidermal cells. Images were taken at indicated timepoints. Scale bar represents 20 µm.