Figure 7.
Microtubule regrowth at centrosomes is impaired in Eml1 mutant conditions. (A) Representative images for pericentrin+ puncta (“centrosomes”) with α-tubulin labeling (“MTs”) on WT and Eml1 cKO cells after ice recovery MT assays (1 and 2 min). (B) Quantifications of percentage (%) of centrosomes exhibiting MT regrowth after 1 or 2 min per analyzed ROI, represented as mean ± SEM. (C) Quantifications for mean length of MTs per centrosome 2 min after ice recovery, represented as mean ± SEM (n = 49 centrosomes for Eml1 cKO and 53 for WT). 16 ROI analyzed from two different cultures after 2 min and 12 ROI analyzed from two different cultures after 1 min. Different cultures are indicated by dots of different colors. Test and significance: Mann-Whitney. P value <0.01 **, <0.001 ***. Scale bars (equivalent for WT and cKO, and for 1 min, 2 min): 10 µm.

Microtubule regrowth at centrosomes is impaired in Eml1 mutant conditions. (A) Representative images for pericentrin+ puncta (“centrosomes”) with α-tubulin labeling (“MTs”) on WT and Eml1 cKO cells after ice recovery MT assays (1 and 2 min). (B) Quantifications of percentage (%) of centrosomes exhibiting MT regrowth after 1 or 2 min per analyzed ROI, represented as mean ± SEM. (C) Quantifications for mean length of MTs per centrosome 2 min after ice recovery, represented as mean ± SEM (n = 49 centrosomes for Eml1 cKO and 53 for WT). 16 ROI analyzed from two different cultures after 2 min and 12 ROI analyzed from two different cultures after 1 min. Different cultures are indicated by dots of different colors. Test and significance: Mann-Whitney. P value <0.01 **, <0.001 ***. Scale bars (equivalent for WT and cKO, and for 1 min, 2 min): 10 µm.

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