Centrosome and α-tubulin modifications at E12.5 and/or E15.5 in WT and Eml1 cKO brains. (A) Analyses of overall numbers of γ-tubulin puncta at each stage. (B and C) γ-tubulin intensity (B) and pcnt dispersion (C) analyses at E12.5 and E15.5 by individual puncta, expressed as mean ± SEM. For centrosome analyses n = 5 individuals from 3 litters were analyzed per genotype and age. Two ROI were analyzed per individual. For pericentrin area analyses: at least four individuals were analyzed from 3 litters per genotype and age. (D) Immunolabeling of α-tubulin on embryonic brain slices at E12.5 in WT and Eml1 cKO. (E and F) Quantifications from the ventricular surface to 100 µm height show a reduction in intensity, especially close to the ventricular surface. Quantification of α-tubulin mean intensity per ROI (47% decrease was observed in the VZ). Values represent mean ± SEM (n = 4 embryos for each case). Test and significance: Mann-Whitney (γ-tubulin and pcnt analyses), Two-way Anova (α-tubulin analyses, data distribution was assumed to be normal but this was not formally tested). P value <0.001 ***, <0.0001 ****. Scale bar (equivalent for WT and cKO): 30 µm.