Eml1 is essential for the recruitment of key proteins at the centrosomes in early corticogenesis. (A) Representative images of immunofluorescence labelling of γ-tubulin at the ventricular surface of embryonic coronal sections at E12.5 from WT and Eml1 cKO individuals. (B) Quantifications of γ-tubulin+ puncta distribution from the 0–70 µm from the apical surface at E12.5 in WT and Eml1 cKO embryonic brain sections, expressed as mean ± SEM. (C) Quantification of γ-tubulin fluorescence intensity at E12.5 (the P value is indicated), expressed as mean ± SEM. (D–F) Similar analyses were performed at E15.5. (G) Representative images of pericentrin labeling at the ventricular surface at E12.5 in WT and Eml1 cKO brains, and quantification of pericentrin dispersion at E12.5, expressed as mean ± SEM. (H) Similar analyses were performed at E15.5 (the P value is indicated). For centrosome analyses n = 5 individuals from 3 litters were analyzed per genotype and age. Two ROI were analyzed per individual. For pericentrin area analyses: at least four individuals were analyzed from 3 litters per genotype and age. Test and significance: Two-way ANOVA, Bonferroni post tests (distribution analyses: data passed normality test), Mann-Whitney. P value <0.05 *. Scale bars (equivalent for WT and cKO): 30 µm (for A and D); 10 µm in G and H (main), 5 µm (for G and H insets).