Centrosomal alterations in human patient and mouse mutant cells. (A) Representative electron microscopy (EM) images of control and EML1 patient cortical progenitors. Cells are untreated or treated with Epothilone D (EpoD). (B) Quantifications (cells with defective centrosomes, black arrowhead in A, and MT aggregates, circled in A) performed on treated or non-treated human cells, values expressed as mean ± SEM. (C) Representative images of pericentrin and γ-tubulin labeling on Pax6+ cells cultured from WT and Eml1 cKO embryonic brains. Enlargement of pericentrin is shown in the right panel and the red contours show the pericentrin areas in WT and Eml1 cKO cells. (D) Quantification of the total number of centrosome puncta per cell and γ-tubulin fluorescence intensity per centrosome represented as mean ± SEM. (E) Quantification of pericentrin fluorescence intensity and pericentrin area represented as mean ± SEM. γ-Tubulin and pericentrin intensity were analyzed in 90 WT and 89 cKO cells from three independent cultures, indicated by different colors. Pericentrin area measurement was performed on 72 WT cells and 73 cKO cells. Test and significance: Mann-Whitney. P value <0.0001 ****. Scale bars: for A 0.2 µm (equivalent for all images); for C (equivalent for WT and cKO): 5 µm (for main and insets).