Figure S4.
Trafficking to the PC is altered in Eml1 cKO RG. (A) Schematized method of the Retention Using Selective Hook (RUSH) approach, used here for PC protein trafficking analyses in primary cultures of Pax6+ cells. (B) Representative images of GM130, Arl13b labeling, and SSTR3-GFP signal on WT and Eml1 cKO cells in culture at the different time points analyzed. (C and D) Quantifications for SSTR3 (C), PKD2 (D) RUSH construct concentration in the Golgi and in the PC over time (0, 30, 60, and 90 min) in WT and Eml1 cKO cells in culture, values represent mean ± SEM. Quantifications were performed on at least 15 cells from two independent cultures for each genotype and analyzed protein. Test and significance: Two-way ANOVA. Sidak’s multiple comparison. Data distribution was assumed to be normal but this was not formally tested. P value <0.05 *, <0.01 **, <0.001 ***. Scale bar (equivalent for WT and cKO, all time points): 5 µm.

Trafficking to the PC is altered in Eml1 cKO RG. (A) Schematized method of the Retention Using Selective Hook (RUSH) approach, used here for PC protein trafficking analyses in primary cultures of Pax6+ cells. (B) Representative images of GM130, Arl13b labeling, and SSTR3-GFP signal on WT and Eml1 cKO cells in culture at the different time points analyzed. (C and D) Quantifications for SSTR3 (C), PKD2 (D) RUSH construct concentration in the Golgi and in the PC over time (0, 30, 60, and 90 min) in WT and Eml1 cKO cells in culture, values represent mean ± SEM. Quantifications were performed on at least 15 cells from two independent cultures for each genotype and analyzed protein. Test and significance: Two-way ANOVA. Sidak’s multiple comparison. Data distribution was assumed to be normal but this was not formally tested. P value <0.05 *, <0.01 **, <0.001 ***. Scale bar (equivalent for WT and cKO, all time points): 5 µm.

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