Eml1 interacting partner analyses reveal centrosomal protein Cep170 as an interactor, and reduced presence at the centrosome in Eml1 mutant cells in vivo. (A) BioID workflow to identify proximal interactors of EML1 and EML1*T243A. (B) For EML1 and EML1*T243A BioID analysis, each hit is represented on the scatter plot displays by its Saint Probability (SP) score versus its fold change in the spectral count over the control. (C) Venn diagram displaying overlapping hits for EML1 and EML1*T243A with an SP ≥ 0.6. (D) Heat map showing the SP scores of EML1 and EML1*T243A proximal interactors. (E) Gene ontology (GO) annotation grouped into biological process of EML1 and EML1*T243A proximal interactors. (F) Proximal interactors of EML1 related to microtubule cytoskeleton, spindle, and organelle cellular components (underlined proteins lose interaction significance in EML1*T243A SP < 0.6). (G) Representative images of Cep170 labeling at the ventricular surface in E12.5 WT and Eml1 cKO brains. (H) Quantifications of Cep170 fluorescence intensity at the centrosomes, also normalized to γ-tubulin intensity, expressed as mean ± SEM (the P value is indicated). (I and J) Similar analyses were performed at E15.5. For BioID experiments each condition has three replicates stemming from three independent experiments. Cep170 fluorescence intensity analyzes were performed from at least four individuals per genotype from three different litters and two ROI analyzed per individual. Test and significance: Mann-Whitney. P value <0.05 *. Scale bars (equivalent for WT and cKO): 10 µm.