Figure S3.
BioID and gene ontology analyses of WT and T243A Eml1 interacting partners, Cep170 cell analyses. (A) Centrosomes were carefully checked in transfected Neuro2A cells, in interphase and during mitosis. No obvious abnormalities were identified. (B and C) Gene ontology (GO) annotation grouped into molecular function (B) and cellular component (C) of EML1 and EML1*T243A proximal interactors. (D) Representative immunoblots of coimmunoprecipitation of EML1 and EML4. Mutant EML1 was not assessed. (E) Analyses of individual puncta of Cep170 fluorescence intensity at the centrosomes, also normalized to γ-tubulin intensity, expressed as mean ± SEM (n = 4 embryos from 2 litters, two ROI analyzed per embryo). Test and significance: Mann-Whitney. P value <0.0001 ****. Scale bar (equivalent all images): 10 µm. Source data are available for this figure: SourceData FS3.

BioID and gene ontology analyses of WT and T243A Eml1 interacting partners, Cep170 cell analyses. (A) Centrosomes were carefully checked in transfected Neuro2A cells, in interphase and during mitosis. No obvious abnormalities were identified. (B and C) Gene ontology (GO) annotation grouped into molecular function (B) and cellular component (C) of EML1 and EML1*T243A proximal interactors. (D) Representative immunoblots of coimmunoprecipitation of EML1 and EML4. Mutant EML1 was not assessed. (E) Analyses of individual puncta of Cep170 fluorescence intensity at the centrosomes, also normalized to γ-tubulin intensity, expressed as mean ± SEM (n = 4 embryos from 2 litters, two ROI analyzed per embryo). Test and significance: Mann-Whitney. P value <0.0001 ****. Scale bar (equivalent all images): 10 µm. Source data are available for this figure: SourceData FS3.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal