Figure 3.
Cell cycle analyses of Pax6+ cells in E12.5 and E15.5 WT and Eml1 cKO brains. (A) Schematic representation of EdU and BrdU injections performed for cell cycle analyses and related formulae to calculate duration of S-phase and the cell cycle. (B) Representative images of EdU/BrdU/Pax6 labeling of E12.5 coronal brain slices from WT and Eml1 cKO. (C) Quantifications of S-phase length (Ts), cell cycle length (Tc), G1+G2+M length in hours at E12.5, expressed as mean ± SEM. (D) Distribution of cells in the E12.5 cortical wall divided into six identical bins. Distributions are shown for cells entering in S-phase, maintained in S-phase and for those that exit S-phase, expressed as mean ± SEM. (E and F) Similar analyses performed at E15.5, comparing mutant cells in the VZ and outside. (G) Schematic representation of cell cycle phase lengths for results obtained at E12.5 and E15.5 in WT and Eml1 cKO embryos. Analysis was performed on five individuals from 3 litters per genotype and two ROI were analyzed per individual. Test and significance: Mann-Whitney, Two-way ANOVA, Dunn’s post test (distribution analyses: data passed normality test). P value <0.05 *, <0.01 **. Scale bars (equivalent for WT and cKO): 15 μm at E12.5 and 30 μm at E15.5.

Cell cycle analyses of Pax6+ cells in E12.5 and E15.5 WT and Eml1 cKO brains. (A) Schematic representation of EdU and BrdU injections performed for cell cycle analyses and related formulae to calculate duration of S-phase and the cell cycle. (B) Representative images of EdU/BrdU/Pax6 labeling of E12.5 coronal brain slices from WT and Eml1 cKO. (C) Quantifications of S-phase length (Ts), cell cycle length (Tc), G1+G2+M length in hours at E12.5, expressed as mean ± SEM. (D) Distribution of cells in the E12.5 cortical wall divided into six identical bins. Distributions are shown for cells entering in S-phase, maintained in S-phase and for those that exit S-phase, expressed as mean ± SEM. (E and F) Similar analyses performed at E15.5, comparing mutant cells in the VZ and outside. (G) Schematic representation of cell cycle phase lengths for results obtained at E12.5 and E15.5 in WT and Eml1 cKO embryos. Analysis was performed on five individuals from 3 litters per genotype and two ROI were analyzed per individual. Test and significance: Mann-Whitney, Two-way ANOVA, Dunn’s post test (distribution analyses: data passed normality test). P value <0.05 *, <0.01 **. Scale bars (equivalent for WT and cKO): 15 μm at E12.5 and 30 μm at E15.5.

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