Figure S1.
Forebrain inactivation of Eml1 leads to subcortical heterotopia in mouse. (A) Schematic representation of the alleles used to generate Eml1 cKO animals. (B) Cresyl violet staining of individual brains at 8 wk showing SH (red outline) in homozygote mutant animals. (C) Representative images of SatB2 immunofluorescence in 3D visualized by light-sheet microscopy in a control E18.5 embryo compared to an Eml1 cKO. No heterotopia can be detected in the control. The homotopic cortex is depicted in transparency (purple) and the heterotopia is shown with a solid rendering (red). Three different angles are shown. (D) Western blot analyses showing that Eml1 expression is lost in P7 cortices. Specific anti-Eml1 antibodies 3E8 are shown in upper panels, and C3GTX in lower panels. Scale bars (equivalent for WT and cKO): 250 µm in B (main); 100 µm in B inset; 500 µm in C. Source data are available for this figure: SourceData FS1.

Forebrain inactivation of Eml1 leads to subcortical heterotopia in mouse. (A) Schematic representation of the alleles used to generate Eml1 cKO animals. (B) Cresyl violet staining of individual brains at 8 wk showing SH (red outline) in homozygote mutant animals. (C) Representative images of SatB2 immunofluorescence in 3D visualized by light-sheet microscopy in a control E18.5 embryo compared to an Eml1 cKO. No heterotopia can be detected in the control. The homotopic cortex is depicted in transparency (purple) and the heterotopia is shown with a solid rendering (red). Three different angles are shown. (D) Western blot analyses showing that Eml1 expression is lost in P7 cortices. Specific anti-Eml1 antibodies 3E8 are shown in upper panels, and C3GTX in lower panels. Scale bars (equivalent for WT and cKO): 250 µm in B (main); 100 µm in B inset; 500 µm in C. Source data are available for this figure: SourceData FS1.

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