γ-Tubulin staining of peroxisome assay and validation of mEos-DCX expression in CGCs. (A) Top: Schematic of peroxisome movement driven by BicD2 or Nesp2-SR48. Bottom: immunocytochemistry with γ-tubulin (cyan) shows that BicD2 gathers peroxisomes at the centrosome, while SR48 distributes peroxisomes throughout the cell soma. (B) Immunocytochemistry of CGCs transfected with mEos-DCX. mEos-DCX (green) is colocalized with acetylated-tubulin (magenta). Scale bars, 5 μm.