Figure S4.
Size increase of peroxisomes does not promote dynein-dominating movements by Nesprin-2. (A) Strategies for increasing peroxisome size. SKL-tagged proteins are imported into the peroxisomes and increase the peroxisome size. GM130C-ter crosslinks multiple peroxisomes through homo-oligomerization of endogenous GRASP65. (B) Quantification of the peroxisome size. COS7 cells were transfected with PEX-GFP-FKBP only (Ctrl), PEX-GFP-FKBP and mVenus-SKL (SKL), or PEX-RFP-FKBP and PEX-mCherry-GM130C-ter (GM130). The area of single peroxisomes was measured. n = 284 (Ctrl), 287 (SKL), and 87 (GM130) peroxisomes from four to six cells per group. Unpaired t test with Welch’s correction. (C) Rapalog-induced peroxisome transport by Nesp2-SR48ΔKASH in COS7 cells expressing mVenus-SKL or PEX-mCherry-GM130C-ter. Representative trajectories are shown with lines of different colors. Bars show mean ± SEM. Scale bars, 2 μm in B; 10 μm in C. Related to Video 7.

Size increase of peroxisomes does not promote dynein-dominating movements by Nesprin-2. (A) Strategies for increasing peroxisome size. SKL-tagged proteins are imported into the peroxisomes and increase the peroxisome size. GM130C-ter crosslinks multiple peroxisomes through homo-oligomerization of endogenous GRASP65. (B) Quantification of the peroxisome size. COS7 cells were transfected with PEX-GFP-FKBP only (Ctrl), PEX-GFP-FKBP and mVenus-SKL (SKL), or PEX-RFP-FKBP and PEX-mCherry-GM130C-ter (GM130). The area of single peroxisomes was measured. n = 284 (Ctrl), 287 (SKL), and 87 (GM130) peroxisomes from four to six cells per group. Unpaired t test with Welch’s correction. (C) Rapalog-induced peroxisome transport by Nesp2-SR48ΔKASH in COS7 cells expressing mVenus-SKL or PEX-mCherry-GM130C-ter. Representative trajectories are shown with lines of different colors. Bars show mean ± SEM. Scale bars, 2 μm in B; 10 μm in C. Related to Video 7.

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