Figure 5.
Kinetics of cargo transport driven by Nesprin-2 SR48. (A) Velocity distribution of peroxisomes transported by Kif5B, BicD2, DHC, and Nesp2-SR48. Velocities from processive transport segments with a distance >1 μm are included. Positive velocities indicate movement toward MT plus ends and negative velocities indicate movement toward MT minus ends. n = 343 (Kif5B), 188 (BicD2), 103 (DHC), 147 (SR48 to MT plus end), and 226 (SR48 to MT minus end) trajectory segments in >25 cells from three to five independent experiments per group. (B and C) Inverse cumulative distribution functions (CDF) of run length toward MT minus end (B) and plus end (C). Lines show the fitted single exponential decay curves and data points show the actual values. (D) Velocity distribution of peroxisomes transported by Nesp2-SR48-LEAA and Nesp2-SR48-5A-AAVV compared to Nesp2-SR48. n = 147 (SR48 to MT plus end), 226 (SR48 to MT minus end), 143 (SR48LEAA), and 82 (SR48-AAVV) trajectory segments in >25 cells from three to five independent experiments per group. (E and F) Inverse cumulative distribution functions (CDF) of run length toward MT minus end (E) and plus end (F). (G) Percentages of plus- and minus-end-directed runs. Unpaired t test with Welch’s correction is used in A and D. Mann Whitney test is used in B, C, E, and F. Bars show mean ± SEM. Numerical values are mean ± SD.

Kinetics of cargo transport driven by Nesprin-2 SR48. (A) Velocity distribution of peroxisomes transported by Kif5B, BicD2, DHC, and Nesp2-SR48. Velocities from processive transport segments with a distance >1 μm are included. Positive velocities indicate movement toward MT plus ends and negative velocities indicate movement toward MT minus ends. n = 343 (Kif5B), 188 (BicD2), 103 (DHC), 147 (SR48 to MT plus end), and 226 (SR48 to MT minus end) trajectory segments in >25 cells from three to five independent experiments per group. (B and C) Inverse cumulative distribution functions (CDF) of run length toward MT minus end (B) and plus end (C). Lines show the fitted single exponential decay curves and data points show the actual values. (D) Velocity distribution of peroxisomes transported by Nesp2-SR48-LEAA and Nesp2-SR48-5A-AAVV compared to Nesp2-SR48. n = 147 (SR48 to MT plus end), 226 (SR48 to MT minus end), 143 (SR48LEAA), and 82 (SR48-AAVV) trajectory segments in >25 cells from three to five independent experiments per group. (E and F) Inverse cumulative distribution functions (CDF) of run length toward MT minus end (E) and plus end (F). (G) Percentages of plus- and minus-end-directed runs. Unpaired t test with Welch’s correction is used in A and D. Mann Whitney test is used in B, C, E, and F. Bars show mean ± SEM. Numerical values are mean ± SD.

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