Both dynein and kinesin-1 are required for nuclear movement in migrating CGCs. (A) Schematic of live imaging set-up of cerebellar lobe culture. Plasmids were electroporated to monitor nuclear movements during neuronal migration. (B–D) Time-lapse sequence of a migrating CGC transfected with DsRed-labeled nuclear-localization-signal (DsRed-NLS; magenta) together with GFP (green; B), GFP-Lis1N (C), or GFP-Kif5B-tail (D). (E) Trajectories of nuclear centroid movements of CGC transfected with GFP (Ctrl; green), GFP-Lis1N (blue), or GFP-Kif5B-tail (orange). (F) Quantitative analyses of CGC nuclear net displacement. n = 21 (Ctrl), 8 (Lis1N), and 17 (Kif5B-tail) neurons from three independent experiments per group. Unpaired t test with Welch’s correction. (G) Schematic illustration of CGC reaggregate culture set-up. (H and I) Representative time-lapse sequence of a CGC (H) and trajectories of nuclear centroid movements (I) from reaggregate cultures. (J) Quantitative analyses of nuclear net displacement. n = 26 (Ctrl), 31 (Lis1N), 27 (p150-CC1), 27 (Kif5B-tail), and 21 (KLC-TPR) neurons from three to four independent experiments per group. Unpaired t test with Welch’s correction was used to compare with Ctrl. Bars show mean ± SEM. Scale bars, 20 μm in A and G; 5 μm in B–D and H. Related to Video 1.