Figure 6.
Hypoosmotic swelling of spheroplasts causes PS exposure.(A) Wild-type and ist2∆ cells (AMY4, MTY47) were spheroplasted in presence of 0.8 M sorbitol. After hypoosmotic shock in 0.1 M sorbitol medium, the spheroplasts were stained with annexin-V that binds to surface exposed PS. (B) Example of cell remnants stained with annexin-V. (C) Quantification of the annexin-V staining.

Hypoosmotic swelling of spheroplasts causes PS exposure. (A) Wild-type and ist2∆ cells (AMY4, MTY47) were spheroplasted in presence of 0.8 M sorbitol. After hypoosmotic shock in 0.1 M sorbitol medium, the spheroplasts were stained with annexin-V that binds to surface exposed PS. (B) Example of cell remnants stained with annexin-V. (C) Quantification of the annexin-V staining.

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