Related to Fig. 7 : STLC and paclitaxel do not cause widespread phosphatidylserine exposure after 24 h, and mitotic inhibitors increased the population of Raji cells in S/G2/M. (A) SKOV3 cells were treated with STLC or paclitaxel for 20 h, then stained with annexin to measure phosphatidylserine exposure as an indicator of apoptosis. As a positive control, cells were treated with 250 μM H₂O₂ to induce apoptosis. Flow cytometry plots show a representative experiment. The graph depicts the percent of annexin-positive cells, with each dot representing an independent replicate. (B) FUCCI Raji cells were treated with STLC or paclitaxel for 20 h, then the samples were run in flow cytometry to verify mitotic inhibition. FUCCI Raji cells will appear mCh⁺ when undergoing mitosis; however, this mCh⁺ population is cumulative of S/G1/M. The mCh⁺ population has been presented in this graph. Data in A and B were compared using one-way ANOVA with Holm–Sidak multiple comparison correction. N = 4 independent experiments. Bars represent the mean ± SEM. **** denotes P < 0.00005.