Figure 6.
Increasing cancer cell adhesion reduces cancer cell phagocytosis. (A) Raji B cells were infected with E-cadherin GFP (CDH1 OE) to induce cell–cell adhesion. Bright-field images show the formation of cell clusters in CDH1-overexpressing cells. (B) Raji B cells expressing H2B-iRFP were opsonized with a CD47 antibody and incubated with mCh-CAAX macrophages for 2 h. The percent of phagocytic macrophages was measured by flow cytometry. Scale bars denote 500 μm. (C) Raji B cells expressing iRFP-CAAX were opsonized with a CD47 antibody and incubated with mCh-CAAX macrophages for 2 h. The percent of iRFP-positive macrophages was measured by flow cytometry. Data were compared using one-way ANOVA with Holm–Sidak multiple comparison correction. N = 4 independent experiments. Bars represent the mean ± SEM. Data collected on the same day are annotated with the same shape point. ** denotes P < 0.005. Refer to the image caption for details.

Increasing cancer cell adhesion reduces cancer cell phagocytosis. (A) Raji B cells were infected with E-cadherin GFP (CDH1 OE) to induce cell–cell adhesion. Bright-field images show the formation of cell clusters in CDH1-overexpressing cells. (B) Raji B cells expressing H2B-iRFP were opsonized with a CD47 antibody and incubated with mCh-CAAX macrophages for 2 h. The percent of phagocytic macrophages was measured by flow cytometry. Scale bars denote 500 μm. (C) Raji B cells expressing iRFP-CAAX were opsonized with a CD47 antibody and incubated with mCh-CAAX macrophages for 2 h. The percent of iRFP-positive macrophages was measured by flow cytometry. Data were compared using one-way ANOVA with Holm–Sidak multiple comparison correction. N = 4 independent experiments. Bars represent the mean ± SEM. Data collected on the same day are annotated with the same shape point. ** denotes P < 0.005.

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