Related to Fig. 5 : Adhesion of SKOV3 limits phagocytosis and validation of integrin knockouts. (A) SKOV3 cells (red) labeled with mCherry-CAAX were incubated with Her2 CAR GFP macrophages (green), and phagocytosis was measured during a 10-h time-lapse. The schematic shows the experimental setup with suspended SKOV3 cells and adherent macrophages, adherent SKOV3 cells and suspended macrophages, or both cells in suspension at the start of the time-lapse. Data were compared using one-way ANOVA with Holm–Sidak multiple comparison correction. N = 3 independent experiments. Bars represent the mean ± SEM. Data collected on the same day are annotated with the same shape point. * denotes P < 0.05. (B) SKOV3 cells were infected with a lentiviral construct encoding puromycin resistance, Cas9, and an sgRNA-targeting ITGAV, ITGB1, ITGB5, or a NT control. After puromycin selection, the polyclonal cell line was stained with an antibody targeting the relevant integrin subunit or an isotype control.