Related to Fig. 3 : Her2 CAR macrophages cause caspase activation in some SKOV3 cells, and Her2 antigen is stripped from apparently viable cells. (A) SKOV3 cells were infected to express the caspase sensor GC3AI, which fluoresces when caspases are active. Her2 CAR or GFP control macrophages were added to the SKOV3 cells, and caspase activity was monitored beginning 24 h later. The graph depicts the percent of SKOV3 cells that activated caspases at any point during a 12-h time-lapse. (B) SKOV3 cells gated based on properties of FSC and SSC were confirmed to be primarily intact viable cells from the absence of propidium iodide. In A, data were compared using one-way ANOVA with Holm–Sidak multiple comparison correction. N = 4 independent experiments. Bars represent the mean ± SEM. Data collected on the same day are annotated with the same shape point. ** denotes P < 0.005.