Related to Fig. 1 : Apoptotic corpses are phagocytosed equivalently in control GFP and Her2 CAR BMDMs. (A) Apoptosis was induced in Jurkats by a 16 h treatment with 0.5 µg/ml Staurosporine. Jurkats were stained with annexin to confirm cell death. Jurkats were washed to remove staurosporine, stained with Hoechst, and then added to Her2 CAR and GFP control macrophages. The percent of macrophages internalizing an apoptotic corpse and the fluorescent intensity of internalized Hoechst were measured by flow cytometry. (B) A representative uncropped image shows the co-incubations presented in Fig. 1 C. Data in A were compared using one-way ANOVA with Holm–Sidak multiple comparison correction. N = 4 independent experiments. Bars represent the mean ± SEM. Data collected on the same day are annotated with the same shape point. **** denotes P < 0.00005. Scale bars in B denote 100 μm. BMDMs, bone marrow–derived macrophages.