Figure S5.
BGN-SBP-mSc localization in mutant cells. (A) Widefield maximum projections of cells stably expressing BGN-SBP-mSc (blue) and immunolabeled for the ERGIC (ERGIC53, green), cis/medial-Golgi (giantin, magenta), and TGN (TGN46, grayscale). Scale bar in main image, 10 µm; and insert, 1 µm. (B and C) Line-scan fluorescence intensity readings across Golgi elements measured at key time points in the RUSH assays shown in Figs. 6 and 7; and Videos 4, 5, 6, 7, 8, and 9. The region measured is shown in inset. Line traces show (B) BGN-SBP-mSc (magenta trace) or (C) SPARC-SBP-mSc accumulates adjacent to ManII-positive Golgi elements (green trace, middle column) and then colocalizes with ManII-BFP (right column). Fluorescence measurements are normalized to the maximal point of each trace to account for differential expression and photobleaching rates of each fluorescent protein. Arrows highlight peak for each protein. ManII, mannosidase II.

BGN-SBP-mSc localization in mutant cells. (A) Widefield maximum projections of cells stably expressing BGN-SBP-mSc (blue) and immunolabeled for the ERGIC (ERGIC53, green), cis/medial-Golgi (giantin, magenta), and TGN (TGN46, grayscale). Scale bar in main image, 10 µm; and insert, 1 µm. (B and C) Line-scan fluorescence intensity readings across Golgi elements measured at key time points in the RUSH assays shown in Figs. 6 and 7; and Videos 4, 5, 6, 7, 8, and 9. The region measured is shown in inset. Line traces show (B) BGN-SBP-mSc (magenta trace) or (C) SPARC-SBP-mSc accumulates adjacent to ManII-positive Golgi elements (green trace, middle column) and then colocalizes with ManII-BFP (right column). Fluorescence measurements are normalized to the maximal point of each trace to account for differential expression and photobleaching rates of each fluorescent protein. Arrows highlight peak for each protein. ManII, mannosidase II.

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