General secretion is differentially impacted by loss of GMAP210 or Golgin-160. (A) Volcano plots showing log-fold change in protein abundance in the media from KO versus WT cultures plotted against significance across three independent replicate experiments. The results from the two golgin KO clonal cell lines were pooled to increase power. Lines and dot colors on the graph are for P < 0.05 and >1 or less than −1 LogFC. Triangular points show hits, which pass an FDR-corrected P < 0.05. (i–iii) Structural ECM proteins (i), ECM regulators (ii), and other proteins of interest (iii) have been highlighted. Proteins significantly changed in the same way in both KO cultures are labeled in dark blue, while proteins impacted in a golgin-specific way are labeled in purple. The proteoglycan family is indicated by green text. (B–H) RT-PCR analysis of the gene expression of (B) BGN, (C) SPARC, (D) DCN, (E) ARHGDIB, (F) FAM20C, (G) PLOD1, and (H) THBS1. Expression levels normalized to the expression of the housekeeping gene YWHAZ. Individual data points represent biological replicate experiments (n = 3–4). Bars show the mean and standard deviation.