Early secretory pathway organization in golgin mutants. (A) Widefield images of MC3T3 cells transfected with Cas9 and gRNA targeting either Trip11 or Golga3 genes and stained for GMAP210 (Trip11) or Golgin-160 (Golga3) to identify KO cells (as indicated by an asterisk) and GM130. Inserts show GM130 label in a WT and a KO cell from a mixed population. Scale bar, 10 µM. (B and C) Confocal maximum projection images of WT and golgin KO cell lines stained for cis/medial-Golgi membrane (giantin, magenta) markers and either ERGIC (ERGIC53, green) (A) or COP1 (βCOP, green) structures (B). Scale bars, 10 µM.