Figure 1.
![The endogenous actin cytoskeleton is fluorescently labeled in the developing TgT2[UbC:Lifeact-EGFP] transgenic quail embryo. (A) Plasmid vectors were used to generate the transgenic line. (B) Tiled confocal images show that the Lifeact-EGFP signal matches the labeling of the actin cytoskeleton by Phalloidin-568 throughout early embryonic development. (C and D) Lifeact-EGFP and Phalloidin-568 labeling in the eye (C) and the otic pit (D). (E) Higher magnification images of non-neural ectoderm demonstrate the similarity of the Lifeact-EGFP signal to staining with Phalloidin-568 and SPY650FastAct.](https://cdn.rupress.org/rup/content_public/journal/jcb/223/9/10.1083_jcb.202404066/3/jcb_202404066_fig1.png?Expires=1771300401&Signature=3~UcSLuztu-5Xiuvt2ngr2Dh5QDUg~TtJ06YOU7ocVavVvfIBmkmAoQyW2Q9VyXSpROP9cevCwV5-xHCwrEMnqBlOUqoKWo3iqNHYehXlF9Eigdzc3Sv5szhkK8GjgzzaMU0WMmIiroIcmtxfqfeAiM6i2t~oCJ2cktEdNSsYIb9Nd1pD1NeJHHQ34bKbbWUFFrnsNFKZUnhZUdwc1dNkn3tnqGAKSwnR-KmyGaHshF27NDL21HwiRFhHkRNDPfnmshwsP3YTYjdpDDjLO91SOKucNjypJ8IVofIavrI7-KCYtOmDkgA9FoYffSupaOb9I3iBp3Zr5eRRMob2Y-7aQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The endogenous actin cytoskeleton is fluorescently labeled in the developing TgT2[UbC:Lifeact-EGFP] transgenic quail embryo. (A) Plasmid vectors were used to generate the transgenic line. (B) Tiled confocal images show that the Lifeact-EGFP signal matches the labeling of the actin cytoskeleton by Phalloidin-568 throughout early embryonic development. (C and D) Lifeact-EGFP and Phalloidin-568 labeling in the eye (C) and the otic pit (D). (E) Higher magnification images of non-neural ectoderm demonstrate the similarity of the Lifeact-EGFP signal to staining with Phalloidin-568 and SPY650FastAct.
