Figure 2.
nekl-4(KD) mutation suppresses B-tubule instability in ccpp-1∆ amphid cilia. (a) Diagram of the amphid cilia indicating the position of the cross-sections examined. MS = middle segment. (b) Examples of microtubule doublet structure in WT, ccpp-1∆, nekl-4(KD), ccpp-1∆; nekl-4(KD), and nekl-4(PEST∆) mutant amphid channels. The WT and ccpp-1∆ panels are reproduced from O’Hagan et al. (2011) and show a male amphid. All other genotypes show a hermaphrodite amphid. Red * = normal microtubule doublet, yellow * = microtubule doublet with broken or missing B-tubule in a representative cilium. Scale bars = 100 nm. (c) Examples of phenotypes seen in nekl-4(KD), ccpp-1∆; nekl-4(KD), and nekl-4(PEST∆) mutant cilia. Scale bars = 100 nm. (d) Table of phenotypes observed in each genotype and numbers of cilia and animals analyzed. The WT and ccpp-1∆ data are reproduced from O’Hagan et al. (2011).

nekl-4(KD) mutation suppresses B-tubule instability in ccpp-1∆ amphid cilia. (a) Diagram of the amphid cilia indicating the position of the cross-sections examined. MS = middle segment. (b) Examples of microtubule doublet structure in WT, ccpp-1∆, nekl-4(KD), ccpp-1∆; nekl-4(KD), and nekl-4(PEST∆) mutant amphid channels. The WT and ccpp-1∆ panels are reproduced from O’Hagan et al. (2011) and show a male amphid. All other genotypes show a hermaphrodite amphid. Red * = normal microtubule doublet, yellow * = microtubule doublet with broken or missing B-tubule in a representative cilium. Scale bars = 100 nm. (c) Examples of phenotypes seen in nekl-4(KD), ccpp-1∆; nekl-4(KD), and nekl-4(PEST∆) mutant cilia. Scale bars = 100 nm. (d) Table of phenotypes observed in each genotype and numbers of cilia and animals analyzed. The WT and ccpp-1∆ data are reproduced from O’Hagan et al. (2011).

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