Figure S5. PIP 3 signaling and the... | Rockefeller University Press

Figure S5.

PIP3signaling and the effect of its perturbation on macropinocytosis. (A) Quantification of GFP-PHPkgE translocation in WT and rasD−rasG− cells in response to FA stimulation (250 μM FA was added at time 0) in the presence of LatA. (B) Quantification of the fluorescent intensity of GFP-PHPkgE at the macropinocytic cups in WT and DKO cells. (C) GFP-PHPkgE translocation in WT and DKO cells in response to FA stimulation (250 μM FA was added at time 0) in the presence of LatA. Top: Time-lapse imaging of translocation. Bottom: Quantification of translocation. (D) Quantification of TRITC-Dextran uptake in WT, pten−, and Dd5P4− cells. (E) Quantification of TRITC-Dextran uptake in WT and DKO cells treated with DMSO or LY294002 (12.5 μM). The plots in A and C show mean and SD; n represents the number of cells analyzed. The plots in B, D, and E show data points with means and SD; n represents the number of cells analyzed. Significance was determined by one-way ANOVA in D and by two-tailed unpaired t test in B, C, and E. Scale bars, 5 μm.

PIP ₃ signaling and the effect of its perturbation on macropinocytosis. (A) Quantification of GFP-PH_PkgE translocation in WT and rasD⁻rasG⁻ cells in response to FA stimulation (250 μM FA was added at time 0) in the presence of LatA. (B) Quantification of the fluorescent intensity of GFP-PH_PkgE at the macropinocytic cups in WT and DKO cells. (C) GFP-PH_PkgE translocation in WT and DKO cells in response to FA stimulation (250 μM FA was added at time 0) in the presence of LatA. Top: Time-lapse imaging of translocation. Bottom: Quantification of translocation. (D) Quantification of TRITC-Dextran uptake in WT, pten⁻, and Dd5P4⁻ cells. (E) Quantification of TRITC-Dextran uptake in WT and DKO cells treated with DMSO or LY294002 (12.5 μM). The plots in A and C show mean and SD; n represents the number of cells analyzed. The plots in B, D, and E show data points with means and SD; n represents the number of cells analyzed. Significance was determined by one-way ANOVA in D and by two-tailed unpaired t test in B, C, and E. Scale bars, 5 μm.