Leep2A and Leep2B form a complex to regulate macropinocytosis. (A) TRITC-Dextran uptake in WT cells expressing GFP or DKO cells expressing GFP, GFP-Leep2A, GFP-Leep2B, or GFP-Leep2A and Leep2B. (B) Quantification of TRITC-Dextran uptake, as shown in A. (C) Quantification of TRITC-Dextran uptake by flow cytometry analysis. (D) Localization of GFP-Leep2A in DKO cells with (top) or without (bottom) the expression of Leep2B. (E) Quantification of TRITC-Dextran uptake in WT cells overexpressing Flag and GFP or Flag-Leep2A and GFP-Leep2B. (F) Quantification of the uptake of Alexa647-Dextran in WT cells overexpressing GFP and RFP or GFP-Leep2A and RFP-Leep2B by flow cytometry analysis. The scatter plots show data points with means and SD; n represents the number of cells analyzed. Significance was determined by one-way ANOVA in B and by two-tailed unpaired t test in E. Scale bars, 5 μm.