Mapping the regions required for complex formation and localization. (A) Top: Schematic representation of full-length Leep2A and truncation constructs. Bottom: Co-IP of GFP, GFP-Leep2A, and GFP-tagged Leep2A truncations with RFP-Leep2B. Fluorescent fusion proteins were expressed in WT cells. IP was performed with RFP-trap, and samples were probed with GFP or RFP antibody. (B) Top: Schematic representation of full-length Leep2B and truncation constructs. Bottom: Co-IP of GFP-Leep2A with RFP, RFP-Leep2B, and RFP-tagged Leep2B truncations. Fluorescent fusion proteins were expressed in WT cells. IP was performed with RFP-trap and samples were probed with GFP or RFP antibody. (C) Localization of GFP-Leep2A or GFP-tagged Leep2A truncations and RFP-Leep2B in WT cells. (D) Localization of GFP-Leep2A and RFP-Leep2B or RFP-tagged Leep2B truncations in WT cells. Scale bars, 5 μm. Source data are available for this figure: SourceData F3.