TLNRD1 in vivo. (A) Efficacy analysis of tlnrd1 CRISPR in zebrafish embryos. The Sanger sequencing chromatograms between control and tlnrd1 sgRNA injected samples were compared using TIDE software. The peak intensities show deviation of sequences and indicate effective editing of the tlnrd1 locus. (B) Zebrafish heart rate analysis. Zebrafish embryos were imaged using fast video microscopy, and heart rate was analyzed using kymographs in Fiji (n > 7 per condition). (C) TLNRD1 expression in human embryos in single cells in various endothelial compartments, fibroblasts, and epithelial cells (data from Xu et al., 2023). (D) TLNRD1 expression (RNA levels) in CCM lesions. Data from Subhash et al. (2019). Controls are four patients diagnosed with temporal lobe epilepsy.