Mass spectrometry analyses identify TLNRD1 as a putative member of the CCM complex. (A–C) Mass spectrometry (MS) analysis of GFP-TLNRD1-binding proteins. A comparison of the GFP-TLNRD1 and GFP datasets is displayed in Venn diagram (A) and volcano plot (B). In the volcano plot, the enrichment ratio (TLNRD1 over GFP) for each protein detected is plotted against the significance of the association (see Table S1 for the MS data). Notably, proteins uniquely identified in either the TLNRD1 or GFP conditions were assigned a fold change of 400 to be displayed on the volcano plot. (C) Proteins specifically enriched to TLNRD1 were mapped onto a protein–protein interaction network (STRING, see the Materials and methods for details). Each node (circle) represents a protein (labeled with gene name), and each edge (line) represents a reported interaction between two proteins. The node’s color indicates the enrichment ratio of that particular protein (TLNRD1 over GFP). The node’s area represents the spectral count of that specific protein in the TLNRD1-GFP dataset. (D) GFP-pulldown in HEK293T cells expressing GFP-TLNRD1 or GFP alone. KRIT1 and ITGB1BP1 recruitment to the bait proteins was then assessed by Western blotting (representative of three biological repeats). Source data are available for this figure: SourceData F1.