High dietary sugar does not impact the levels of H3K18ac and H3K27ac in the PCs. (A–B′) Compared with that observed in the PCs of ND-fed flies (A and A′), the levels of H3K18ac (red) remain unaltered in the PCs of HSD-fed flies (B and B′). Arrows mark the PCs in A and B. Phalloidin (green) marks the cardiac tube and the alary muscles. DAPI marks the nuclei in A′ and B´. (C) Quantification of the mean fluorescence intensity for H3K18ac in the PCs of ND- and HSD-fed flies. The dots represent the number of PCs analyzed for each genotype. (D–E′) Compared with that observed in the PCs of ND-fed flies (D and D′), the levels of H3K27ac (red) remain unaltered in the PCs of HSD-fed flies (E and E′). Arrows mark the PCs in D and E. Phalloidin (green) marks the cardiac tube and the alary muscles. DAPI marks the nuclei in D′ and E′. (F) Quantification of the mean fluorescence intensity for H3K27ac in the PCs of ND- and HSD-fed flies. The dots represent the number of PCs analyzed for each genotype. (G) Feeding on HSD led to an increase in the levels of upd3 expression in the PCs of the flies of all the genotypes mentioned. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. Genotypes are as mentioned. Data are represented as mean ± SD. P values (ns ≥ 0.05, ***P < 1 × 10−3) were obtained by unpaired Student’s t test (two-tailed) with Welch’s correction (C and F), by two-way ANOVA with Tukey’s multiple-comparison test (G). Scale bars, 50 μm.
