Attenuation of FAO in the PCs rescue the elevated levels of upd3 and prc expressions as well as abnormal cardiac function associated with high dietary sugar. (A) Feeding on HSD led to an increase in the levels of upd3 expression in the PCs of flies of all the genotypes mentioned. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. (B) Increase in the levels of prc expression in fat cells of the flies of the genotypes mentioned when reared on HSD. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. (C–E) Changes in heart period (C), diastolic interval (D), and systolic interval (E) upon either knocking down or knocking out whd in the PCs of HSD-fed flies. The dots represent the samples analyzed for each genotype. (F) Levels of expression of different enzymes involved in FAO in the PCs of ND- and HSD-fed flies upon attenuating JNK pathway in the PCs. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. (G) Increase in the levels of upd3 expression in the PCs of flies of the genotypes mentioned when reared on HSD. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. Genotypes are as mentioned. Data are represented as mean ± SD. P values (ns ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 1 × 10−3) were obtained by unpaired Student’s t test (two-tailed) with Welch’s correction (F), by one-way ANOVA with Tukey’s multiple comparison test (C, D, and E), and by two-way ANOVA with Tukey’s multiple-comparison test (A, B, and G).
