The PCs of HSD-fed flies exhibit altered lipid metabolism. (A and B) Increase in the levels of lipid accumulation (LipidTOX staining; red) in the PCs of HSD-fed flies (B) as compared with those reared on ND (A). (C) Quantification of the mean fluorescence intensity for LipidTOX staining in the PCs flies reared on either ND or HSD. The dots represent the number of PCs analyzed for each genotype. (D and E) Increase in the reporter YFP expression for CG3902 in the PCs of HSD-fed flies (E) as compared to those fed on ND (D). Phalloidin (red) marks the cardiac tube and the alary muscles. (F) Quantification of the mean fluorescence intensity for CG3902-YFP expression in the PCs of flies reared on either ND or HSD. The dots represent the number of PCs analyzed for each genotype. (G) Increase in the levels of upd3 expression in the PCs of flies of the genotypes mentioned when reared on HSD. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. (H) Feeding on HSD leads to an increase in the levels of upd3 expression in the PCs of flies of the genotypes mentioned. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. Genotypes are as mentioned. Data are represented as mean ± SD. P values (***P < 1 × 10−3) were obtained by unpaired Student’s t test (two-tailed) with Welch’s correction (C and F) and by two-way ANOVA with Tukey’s multiple-comparison test (G and H). Scale bars, 50 μm.
