The PCs of HSD-fed flies exhibit low sugar uptake and compromised glycolysis. (A and B) Drop in the levels of glucose uptake (2-NBDG staining; green) in the PCs of HSD-fed flies (B) as compared to those reared on ND (A). (C) Quantification of the mean fluorescence intensity for 2-NBDG in the PCs of flies reared on either ND or HSD. The dots represent the number of PCs analyzed for each genotype. (D and E) Drop in the reporter GFP expression (green) for Glut1 in the PCs of HSD-fed flies (E) as compared with those reared on ND (D). (F) Quantification of the mean fluorescence intensity for Glut1-GFP expression in the PCs of flies reared on either ND or HSD. The dots represent the number of PCs analyzed for each genotype. (G and H) Drop in the reporter GFP expression for HexA in the PCs of HSD-fed flies (H) as compared with those reared on ND (G). (I) Quantification of the mean fluorescence intensity for HexA-GFP expression in the PCs of flies reared on either ND or HSD. The dots represent the number of PCs analyzed for each genotype. (J and K) Drop in the reporter GFP expression for pgi in the PCs of HSD-fed flies (K) as compared with those reared on ND (J). (L) Quantification of the mean fluorescence intensity for pgi-GFP expression in the PCs of flies reared on either ND or HSD. The dots represent the number of PCs analyzed for each genotype. Genotypes are as mentioned. Data are represented as mean ± SD. P values (***P < 1 × 10−3) were obtained by unpaired Student’s t test (two-tailed) with Welch’s correction (C, F, I, and L). Scale bars, 50 μm.
