Flies fed on high dietary sugar exhibit hallmark features of T2D and excessive prc expression. (A) Increase in hemolymph trehalose levels in flies reared on HSD compared with those reared on ND on day 20AE. (B) Increased levels of TAG in flies reared on HSD on day 20AE. (C and C′) Changes in the levels of p-Akt in flies reared on ND and HSD with or without insulin treatment. (D) Increase in the levels of dilp2, dilp3, and dilp5 expressions in flies reared on HSD compared with those reared on ND on day 20AE. (E) Survival rates of flies reared on either ND or HSD. Flies fed on HSD exhibited reduced lifespan. (F) The median lifespan of HSD-fed flies was 7 days shorter than that of the flies reared on ND. (G) Feeding on HSD led to an increase in the levels of prc expression in the PCs of the flies of all the genotypes mentioned. The transcript levels are normalized to that of the constitutive ribosomal gene rp49. Genotypes are as mentioned. Data are represented as mean ± SD. P values (*P < 0.05, **P < 0.01, ***P < 1 × 10−3) were obtained by unpaired Student’s t test (two-tailed) with Welch’s correction (A, B, C′, D, and F), by two-way ANOVA with Tukey’s multiple-comparison test (G), and curve comparison was performed using Log-Rank (Mantel-Cox) test (E). Source data are available for this figure: SourceData FS1.
