Figure S3.
Similar ploidy distributions in wildtype, E2F7
Q206X
, and E2F8Q462XHep-Org lines. Quantification of DAPI signal in wildtype, E2F7Q206X, and E2F8Q462X Hep-Org lines grown in culture medium or after 3 days of CHIR99021 removal. Organoids were grown in 3D, trypsinized, cytospun onto slides, fixed, and stained with DAPI. DAPI signal was normalized to the median (black line) of each sample (between 350 and 500 cells analyzed per sample, N = 2 experiments, ns = not significant, Mann–Whitney test).