Figure 3.
Membrane association of membrane anchoring proteins is lost during cleavage regression in endomitosis. (A–D) Representative stills of IF experiments showing DAPI, α-tubulin, E-cadherin, and membrane-anchoring protein (A) RacGAP1, (B) Anillin, (C) SEPT9, or (D) CIT-K in late anaphase and late telophase, with either ingressed or regressed cleavage furrows. Hep-Org 1 line expressing E-cadherin-tdTomato was used for IF stainings. Scale bars represent 3 µm. Close-ups show single- or double-channel images of marked regions of interest (white box). Dashed lines represent membrane outline. All images show one plane in the middle of the midbody. Numbers in the top right show quantification of displayed localization. More detailed quantifications of RacGAP1, Anillin, SEPT9, and CIT-K localizations during the different stages are shown in Fig. 4.

Membrane association of membrane anchoring proteins is lost during cleavage regression in endomitosis. (A–D) Representative stills of IF experiments showing DAPI, α-tubulin, E-cadherin, and membrane-anchoring protein (A) RacGAP1, (B) Anillin, (C) SEPT9, or (D) CIT-K in late anaphase and late telophase, with either ingressed or regressed cleavage furrows. Hep-Org 1 line expressing E-cadherin-tdTomato was used for IF stainings. Scale bars represent 3 µm. Close-ups show single- or double-channel images of marked regions of interest (white box). Dashed lines represent membrane outline. All images show one plane in the middle of the midbody. Numbers in the top right show quantification of displayed localization. More detailed quantifications of RacGAP1, Anillin, SEPT9, and CIT-K localizations during the different stages are shown in Fig. 4.

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