Absence of RIF1-positive ultrafine DNA bridges in late anaphase and telophase. Representative images of RIF1 staining in Hep-Org 1 expressing E-cadherin-tdTomato. RIF1 can be detected on ultrafine DNA bridges during early anaphase (left image, white arrow) but is absent in all late anaphase and telophase structures (right). Hep-Org 1 line expressing E-cadherin-tdTomato was grown in 3D, fixed, and stained for DAPI (gray) and RIF1 (magenta). Scale bars represent 3 µm. Numbers in the top right corner indicate the number of cells with RIF1-positive ultrafine DNA bridges during early anaphase (left) and the number of cells with no RIF1 staining in late anaphase or telophase (right).