Figure S4.
Classification of TMT from wild-type and poc1Δ, the BC inner junction in the core region does not contain Poc1, and the inner scaffold is a stack of circular rings in Tetrahymena. (A) Subtomogram averages and classifications of the wild-type and poc1Δ TMT from the core region. Note the loss of C-tubules in wild-type TMTs, indicating C-tubules are labile in the core region of the BB. The number of subtomograms in each class is indicated. Classes with black boxes around them indicate the complete TMT structure from wild-type and poc1Δ. (B) Cross-sectional view of the complete TMT from the wild-type core region zoomed in on the B–C inner junction. Arrowheads point to the B–C inner junction structure (yellow) and the structure of the B–C inner junction attachment to the inner scaffold (marigold). (C) Comparison of wild-type Tetrahymena TMTs from the proximal (salmon) and core (blue) regions. The overlay reveals the B–C inner junction is different in architecture between the proximal and core regions. (D) Overlay of complete wild-type (gray) and poc1Δ (green) TMTs from the core region in cross-section (left) and luminal side view (right). Although the B–C inner junction inner scaffold attachment site is lost in poc1Δ, the structure of the B–C inner junction itself is not different between wild-type and poc1Δ. Also note that the C-tubule links to the B-tubule using apparently non-tubulin structures that are retained in complete poc1Δ TMTs. (E) The Tetrahymena inner scaffold is a series of stacked rings. Left, the structure of the inner scaffold backfit to the BB core region. Middle, projection of the density onto the X-Z plane. Right, Fourier transform of the projected density (top) and a linescan of intensity at 8 nm layer line (bottom) indicates its Bessel order close to zero, suggesting the scaffold is a zero-start helix. See Materials and methods for a description of this analysis. (F) Map of locations of complete TMT subtomograms (green) versus partial TMT subtomograms (red) in the proximal region of poc1Δ BBs, eight representative BBs depicted. Note that complete TMT subtomograms tend to cluster together on the same TMT.

Classification of TMT from wild-type and poc1Δ, the BC inner junction in the core region does not contain Poc1, and the inner scaffold is a stack of circular rings in Tetrahymena. (A) Subtomogram averages and classifications of the wild-type and poc1Δ TMT from the core region. Note the loss of C-tubules in wild-type TMTs, indicating C-tubules are labile in the core region of the BB. The number of subtomograms in each class is indicated. Classes with black boxes around them indicate the complete TMT structure from wild-type and poc1Δ. (B) Cross-sectional view of the complete TMT from the wild-type core region zoomed in on the B–C inner junction. Arrowheads point to the B–C inner junction structure (yellow) and the structure of the B–C inner junction attachment to the inner scaffold (marigold). (C) Comparison of wild-type Tetrahymena TMTs from the proximal (salmon) and core (blue) regions. The overlay reveals the B–C inner junction is different in architecture between the proximal and core regions. (D) Overlay of complete wild-type (gray) and poc1Δ (green) TMTs from the core region in cross-section (left) and luminal side view (right). Although the B–C inner junction inner scaffold attachment site is lost in poc1Δ, the structure of the B–C inner junction itself is not different between wild-type and poc1Δ. Also note that the C-tubule links to the B-tubule using apparently non-tubulin structures that are retained in complete poc1Δ TMTs. (E) The Tetrahymena inner scaffold is a series of stacked rings. Left, the structure of the inner scaffold backfit to the BB core region. Middle, projection of the density onto the X-Z plane. Right, Fourier transform of the projected density (top) and a linescan of intensity at 8 nm layer line (bottom) indicates its Bessel order close to zero, suggesting the scaffold is a zero-start helix. See Materials and methods for a description of this analysis. (F) Map of locations of complete TMT subtomograms (green) versus partial TMT subtomograms (red) in the proximal region of poc1Δ BBs, eight representative BBs depicted. Note that complete TMT subtomograms tend to cluster together on the same TMT.

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